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10x Genomics
Chromium Single Cell Immune Profiling

Cell Ranger2.0, printed on 11/05/2024

V(D)J Annotations

cellranger vdj pipeline produces V(D)J annotations on the assembled contigs and on the clonotype consensus sequences in multiple formats.

File type overview

File type | Description

  •     |-

CSV | High-level annotations with one contig, consensus, or clonotype per row. JSON | Detailed annotations, including alignment coordinates and amino acid translations. BED | Germline V(D)J segments as features, for use with a tool like IGV.


Annotation files

File | Description

  •                                 |-

clonotypes.csv | High-level descriptions of each clonotype. consensus_annotations.{csv,json} | High-level and detailed annotations of each clonotype consensus sequence. filtered_contig_annotations.csv | High-level annotations of each high-confidence, cellular contig. This is a subset of all_contig_annotations.csv. all_contig_annotations.{csv,bed,json} | High-level and detailed annotations of each contig.


Clonotype CSV file (clonotypes.csv)

Column Description
clonotype_id The ID of the clonotype to which this consensus sequence was assigned.
frequency The observed number of cell-barcodes with this clonotype.
proportion The observed fraction of cell-barcodes with this clonotype.
cdr3s_aa A semicolon-delimited list of chain:sequence pairs, where "chain" is e.g., TRA or TRB and "sequence" is the CDR3 amino acid sequence for that chain.
cdr3s_nt A semicolon-delimited list of chain:sequence pairs, where "chain" is e.g., TRA or TRB and "sequence" is the CDR3 nucleotide sequence for that chain.

Contig annotation CSV files (*contig_annotations.csv)

name description
barcode Cell-barcode for this contig.
is_cell True/False value indicating whether the barcode was called as a cell.
contig_id Unique identifier for this contig.
high_confidence True/False value indicating whether the contig was called as high-confidence (unlikely to be a chimeric sequence or some other artifact).
length The contig sequence length in nucleotides.
chain The chain associated with this contig; e.g., "TRA" or "TRB". A value of "Multi" indicate that segments from multiple chains were present.
v_gene The highest-scoring V segment, e.g., TRAV1-1.
d_gene The highest-scoring D segment, e.g., TRBD1.
j_gene The highest-scoring J segment, e.g., TRAJ1-1.
c_gene The highest-scoring C segment, e.g., TRAC.
full_length The sequence spans the 5′ end of V to the 3′ end of J.
productive True/False/None value indicating whether the transcript is predicted to translate to a protein with a CDR3 region. "None" indicates that the contig does not span the 5′ end of a V region to the 3′ end of a J region, and so the produtivity of the transcript could not be determined. In addition to being V-J spanning, the sequence must have a detectable CDR3 region, have a start codon in the expected part of the V sequence, and have no stop codons in the V-J region.
cdr3 The predicted CDR3 amino acid sequence.
cdr3_nt The predicted CDR3 nucleotide sequence.
reads The number of reads aligned to this contig.
umis The number of distinct UMIs aligned to this contig.
raw_clonotype_id The ID of the clonotype to which this cell-barcode was assigned.
raw_consensus_id The ID of the consensus sequence to which this contig was assigned.

Consensus annotation CSV files (consensus_annotations.csv)

Column Description
clonotype_id The ID of the clonotype to which this consensus sequence was assigned.
consensus_id The ID of this consensus sequence.

The remaining columns are shared with those under the "Contig annotation CSV files" section.