10x Genomics
Visium Spatial Gene Expression

Space Ranger1.2, printed on 12/27/2024

Targeted Gene Expression Overview

Targeted Gene Expression technology focuses on a selected panel of relevant targeted genes which are directly enriched from a Visium spatial RNA-seq library prior to sequencing. Specific enrichment of the targeted molecules of interest by hybridization-capture enables Targeted Gene Expression to provide enhanced information about the expression of targeted genes at significantly lower sequencing depths. Targeted Gene Expression is fully compatible with Visium and relies on the same underlying Space Ranger analysis software as untargeted spatial gene expresion..

Resources and additional information are available below:

• For background information on Visium Targeted Gene Expression see the Targeted Gene Expression Glossary
• To learn how to use Space Ranger with Visium Targeted Gene Expression data see Targeted Gene Expression Analysis
• For more information on the pre-designed gene panels available for Visium Targeted Gene Expression see Pre-Designed Panels for Targeted Gene Expression
• To generate a new customized target gene panel for Targeted Gene Expression see the 10x Genomics Custom Panel Designer
• To learn how to compare targeted data with its parent whole transcriptome data see the targeted-compare pipeline
• To learn how to estimate recommended sequencing depths for Visium targeted data from an existing whole transcriptome analysis dataset see the new Space Ranger command targeted-depth