What is Feature Barcoding?Feature Barcoding AnalysisCRISPR OutputsAntibody OutputsCell Ranger3.0, printed on 11/05/2024
Cell Ranger computes sequencing quality and application results metrics on each supported library, which currently are Antibody Capture, CRISPR Guide Capture, and Custom (user-defined assays that are neither Antibody nor CRISPR). These metrics will be computed and displayed only when one of these library types was used. This page describes Antibody Capture libraries metrics for QC of the library prep and sequencing of antibody libraries, which appear in the metrics_summary.csv file and on the web_summary.html page.
| Metric | Description |
|---|---|
Antibody: Number of Reads | Total number of reads. |
Antibody: Mean Reads per Cell | The total number of reads divided by the number of barcodes associated with cell-containing partitions. |
Antibody: Valid Barcodes | Fraction of reads with a cell-barcode found in or corrected to one that is found in the whitelist. |
Antibody: Fraction Reads in | Fraction of reads lost after removing barcodes with unusually high UMI counts, which may have been formed by antibody aggregates. Applies only to reads in "Antibody Capture" input library. |
Antibody: Sequencing Saturation | Fraction of antibody library reads originating from an already-observed UMI. This is a function of library complexity and sequencing depth. More specifically, this is a ratio where: the denominator is the number of reads with a recognized antibody barcode, valid cell-barcode, and valid UMI, and the numerator is the subset of those reads that had a non-unique combination of (cell-barcode, UMI, antibody barcode). |
Antibody: Q30 Bases in Barcode | Fraction of cell barcode bases with Q-score >= 30, excluding very low quality/no-call (Q <= 2) bases from the denominator. |
Antibody: Q30 Bases in Antibody Read | Fraction of bases from the read containing the antibody barcode with Q-score >= 30, excluding very low quality/no-call (Q <= 2) bases from the denominator. This is Read 2 for the Single Cell 3' v3 and Single Cell 5' chemistries. |
Antibody: Q30 Bases in Sample Index | Fraction of sample index bases with Q-score >= 30, excluding very low quality/no-call (Q <= 2) bases from the denominator. |
Antibody: Q30 Bases in UMI | Fraction of UMI bases with Q-score >= 30, excluding very low quality/no-call (Q <= 2) bases from the denominator. |
Antibody: Fraction Antibody Reads | Fraction of reads that contain a recognized antibody barcode |
Antibody: Fraction Antibody | Fraction of reads that contain a recognized antibody barcode, a valid UMI, and a cell-associated barcode. |
Antibody: Antibody Reads | Number of antibody reads usable divided by the number of cell-associated barcodes. |
Antibody: Fraction Unrecognized | Among all reads, the fraction with an unrecognizable antibody barcode |
Antibody: Antibody Reads in Cells | Among reads with a recognized antibody barcode, a valid UMI, and a valid barcode, the fraction associated with cell-containing partitions. |
Antibody: Median UMIs per Cell | Median UMIs per Cell (summed over all recognized antibody barcodes). |