Cell Ranger ARC2.0, printed on 12/27/2024
The atac_peaks.bed file contains the output of the [peak-calling algorithm][2]. Each peak is an interval on the genome that has a local enrichment of transposase cut-sites. The peaks file reports those intervals in [BED][1] format. Cell Ranger ARC produces peaks whose coordinates are numerically sorted and non-overlapping and whose chromosomes are sorted in the same order as the reference genome. Additionally, header lines beginning with # record information about the sample, the reference used and primary contigs in the reference.
The set of called peaks reflected in this file form the set of primary 'features' that are measured for each cell. Each peak has a corresponding row in the feature-barcode [matrix output files][3].
See the [Algorithm Overview][2] page for details on how Cell Ranger ARC produces peaks.
Note: cellranger-arc count can be run with a user-specified BED file containing peaks using the --peaks argument as described here
| Column Number | Name | Description |
|---|---|---|
| 1 | chrom | Reference genome chromosome of peak |
| 2 | chromStart | Start position of peak on chromosome. |
| 3 | chromEnd | End position of peak on chromosome. The end position is exclusive, so represents the position immediately following the peak interval. |
[1]: https://genome.ucsc.edu/FAQ/FAQformat.html#format1 "BED Format" [2]: ../algorithms/overview#atac_peaks "Peak-Calling Algorithm" [3]: matrices "Matrix Output Files"