10x Genomics
Chromium Single Cell Immune Profiling

Cell Ranger3.1, printed on 11/05/2024

What is Feature Barcode Technology for Immune Profiling?

Feature Barcode technology is a method for adding extra layers of information to cells by running single-cell gene expression in parallel with other assays. For example, Feature Barcode technology enables cell surface protein measurement using antibodies and antigen specificity using barcoded pMHC multimers. A comprehensive list of Feature Barcode compatible products and partners can be found here.

Cell Ranger processes Feature Barcode data for 5' Single Cell Immune Profiling solution similar to 3' Single Cell Gene Expression solution. The following pages take you to the relevant pages for analyzing Feature Barcode data. While these pages are hosted on 3' Single Cell solution's section, they are equally applicable to 5' Immune Profiling solution:

• For Feature Barcode terminlogy see the Feature Barcode Glossary.
• To learn how to use Cell Ranger with Feature Barcode data see Feature Barcoding Analysis with Gene Expression and Feature Barcoding Only Analysis.
• To learn how to view Feature Barcoding data in Loupe Browser, see the Using Loupe Browser with Feature Barcode technology.
• For an additional tutorial on how to perform analysis with multiple library types (Single Cell V(D)J + 5′ Gene Expression + Feature Barcode technology), see the Integrated Analysis.
• To learn how to describe Feature Barcode reagents see Feature Barcode Reference.