10x Genomics
Chromium Single Cell Gene Expression

Cell Ranger7.1, printed on 12/25/2024

Feature Barcode Overview

Feature Barcode technology is a method for adding extra layers of information to cells by running single cell gene expression in parallel with other assays.

Feature Barcode technology compatible with the 3' Chromium Next GEM Single Cell Gene Expression assay:

• Antibody Capture (cell surface protein measurement): A method to label cell surface proteins using a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide (e.g. BioLegend TotalSeq™–B Antibodies).
• CRISPR: A method to analyze gene expression changes caused by the presence of CRISPR perturbations. The assay captures the full transcriptome and transfected guide RNAs from the same cell to correlate changes in the transcriptome to CRISPR perturbations.
• 3' Cell Multiplexing: A method to label a given cell (or nuclei) sample with a molecular tag and subsequently pooling this sample with other labeled samples. Please see the What is Cell Multiplexing? page for assay and data analysis information.

Visit the relevant pages for analyzing and understanding Antibody Capture or CRISPR Guide Capture data:

• For background on Feature Barcode assay schemes and terminology, see the Feature Barcode Glossary.
• To learn how to use Cell Ranger with Feature Barcode data, see the Feature Barcode Analysis page for Antibody Capture or CRISPR Guide Capture.
• To learn how to describe your Feature Barcode reagents as inputs to the cellranger count pipeline, visit Feature Barcode Reference page.
• To learn how to view Feature Barcode data in Loupe Browser, see the Feature Barcode Tutorial.
• For assay-related resources (User Guides, Demonstrated Protocols, Technical Notes, SDS), see Gene Expression Feature Barcode Technology.