10x Genomics
Chromium Single Cell Gene Expression

Cell Ranger7.1, printed on 12/25/2024

Targeted Gene Expression Overview

Targeted Gene Expression technology focuses on a selected panel of relevant targeted genes which are directly enriched from a Chromium single cell gene expression library prior to sequencing. Specific enrichment of the targeted molecules of interest by hybridization-capture enables Targeted Gene Expression to provide enhanced information about the expression of targeted genes at significantly lower sequencing depths than Whole Transcriptome Analysis (WTA). Targeted Gene Expression is fully compatible with both the Chromium 3' and 5' single cell gene expression solutions (including Feature Barcode Technology), and relies on the same underlying Cell Ranger analysis software.

Resources and additional information are available below:

• Targeted Gene Expression glossary
• Targeted Gene Expression analysis with Cell Ranger
• Understanding Targeted Gene Expression analysis outputs
• Pre-designed gene panels (human only)
• Design a custom panel
• 10x Genomics custom gene panel designer
• Compare targeted data to whole transcriptome data
• Estimate recommended sequencing depths for targeted data